Detection of soy proteins in processed foods using a novel enzyme-linked immunosorbent assay with a polyclonal antibody against Gly m 5

To enhance the accuracy of allergen labeling, we developed and optimized an enzyme-linked immunosorbent assay (ELISA) employing both polyclonal antibodies to Gly m 5 and an advanced extraction buffer to detect soy proteins in several food matrices. The developed method demonstrated improved sensitivity and specificity, and achieved a limit of detection and a limit of quantification of 0.011 μg of soy protein/g and 0.015 μg of soy protein/g, respectively. The recoveries from soy-protein-spiked food samples ranged from 65% to 119%, while the coefficients of reproducibility and variation for repeatability remained below 2.7% and 1.4%, respectively. This method provides a reliable approach for reducing allergen exposure risks and verifying food label claims. • A unique ELISA was developed for the detection of soy protein. • This novel ELISA utilizes polyclonal antibodies and a proprietary extraction buffer. • It exhibits high specificity with minimal cross-reactivity to tested food matrices. • The ELISA demonstrates good sensitivity and high recovery rates. • The assay can detect hidden soy protein contamination in food products.