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April 22, 2026Microbiology SpectrumOpen Access

An integrated molecular approach for the detection and species-level identification of Leishmania spp. in clinical samples

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Authors

MRMarikena G. RissoWGW. GarcíaJLJuan José Lauthier

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Overview

Demonstrates enhanced species-level identification of Leishmania in clinical samples, suggesting improved diagnostic accuracy and management in endemic regions.

Key Points

  • The aim is to develop a molecular approach for accurate detection and species identification of Leishmania in clinical samples.
  • Utilized a standardized molecular workflow combining kDNA amplification and hsp70 gene sequencing.
  • Evaluated with 150 clinical samples from 83 patients to assess sensitivity and specificity.
  • Compared performance against microscopy for differential diagnosis.
  • Achieved 69.7% sensitivity and 68% specificity, outperforming microscopy.
  • Identified four species of Leishmania in Argentina through sequencing.
  • Demonstrated capability for detecting low parasite loads down to 0.05 parasites/mL.

Cite This Study

Risso et al. (2026) studied this question.

synapsesocial.com/papers/69e865d76e0dea528ddea5bbhttps://doi.org/10.1128/spectrum.03665-25
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