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April 23, 2026Biochemistry and Biophysics Reports0 citationsOpen Access

Electromyostimulation Effects on Sarcolipin and Calcium Signaling in Rat Skeletal Muscles

Effects of electromyostimulation on sarcolipin and Ca2+ signaling in rat skeletal muscles

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Why the study?

Expression of Ca2+ regulators is modified by resistance training, suggesting that Ca2+ signaling may be altered during a period of resistance training.

Does repeated transcutaneous electromyostimulation alter sarcolipin and Ca2+ signaling expression in male Sprague-Dawley rats?

Population

Male Sprague-Dawley rats

Comparison

Exercised legs vs control legs

Design

Animal experimental study

Follow-up

3 h after the 1st, 5th, and 10th exercise sessions

Key result

Repeated electromyostimulation significantly increased sarcolipin and RCAN1 expression in exercised rat legs compared to control legs after the 5th and 10th sessions (p<0.001).

Authors

KOKoki OkumuraJTJunya TakegakiKSKohei Sase

Discussion

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Overview

Should not change clinical practice; leaves open SLN/RCAN1 role in resistance training muscle adaptations.

Key Points

  • This study investigates how electromyostimulation affects sarcolipin expression and calcium signaling in rat skeletal muscles.
  • Male Sprague-Dawley rats underwent repeated resistance exercise via transcutaneous electromyostimulation.
  • Expression of calcium-related factors was measured 3 hours after the 1st, 5th, and 10th exercise sessions.
  • Sarcolipin expression was significantly higher in exercised legs after the 5th (p < 0.001) and 10th sessions (p < 0.001).
  • Calcineurin showed significant effects from exercise (p = 0.009) and session (p < 0.001).
  • Pearson's correlation indicated a significant relationship between sarcolipin and RCAN1 (r = 0.630, p = 0.005).

Structured PICO

Does repeated transcutaneous electromyostimulation alter sarcolipin and Ca2+ signaling expression in male Sprague-Dawley rats?

P
Population
Male Sprague-Dawley rats
I
Intervention
Repeated resistance exercise via transcutaneous electromyostimulation
C
Comparator
Control legs
O
Outcome
Expression of Ca2+-related factors (sarcolipin, RCAN1, calcineurin, CaMKII, SERCA) 3 h after the 1st, 5th, and 10th exercise sessionssurrogate

Repeated electromyostimulation increases sarcolipin expression in rat skeletal muscle, which correlates with enhanced Ca2+ signaling markers.

Main Result

p-value: p=<0.001

Cite This Study

Okumura et al. (2026) studied Healthy rats. Transcutaneous electromyostimulation vs. Control legs was evaluated on Expression of sarcolipin (SLN) and regulator of calcineurin 1 (RCAN1) (p=<0.001). Repeated electromyostimulation significantly increased sarcolipin and RCAN1 expression in exercised rat legs compared to control legs after the 5th and 10th sessions (p<0.001).

synapsesocial.com/papers/69e9b89b85696592c86ebc03https://doi.org/10.1016/j.bbrep.2026.102588
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