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April 23, 2026AJP Heart and Circulatory Physiology

Cardiac Metabolic Remodeling Drives Dicarbonyl Stress-Induced Mitochondrial Dysfunction in Experimental Heart Failure with Preserved Ejection Fraction

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Why the study?

Although metabolic remodeling and mitochondrial dysfunction are central features of HFpEF, the direct mechanistic link between altered cardiac metabolism and mitochondrial impairment remains elusive.

Population

8-10-week-old male and female mice

Comparison

Infusion of angiotensin-II and phenylephrine

Design

Preclinical animal study

Key result

Cardiac metabolic remodeling in experimental HFpEF drove a 7-fold increase in mitochondrial protein glycation and a ~20% reduction in mitochondrial calcium retention capacity.

Authors

AAAnkit AryalPMParnia MobasheranLBLuther Bishop

Discussion

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Member takes

Overview

Should not yet change HFpEF practice; leaves open glycative stress as targetable mitochondrial pathway for future trials.

Key Points

  • To explore the relationship between cardiac metabolic remodeling and mitochondrial dysfunction in heart failure with preserved ejection fraction (HFpEF).
  • Infused angiotensin-II and phenylephrine in mice to induce HFpEF characteristics.
  • Conducted metabolomic analysis to assess changes in mitochondrial metabolism.
  • Measured mitochondrial function and protein glycation levels using mass spectrometry.
  • Mice exhibited preserved ejection fraction with impaired diastolic function and reduced physical endurance.
  • Mitochondrial respiration and complex II abundance significantly decreased, and there was a marked increase in mitochondrial protein glycation.
  • Dicarbonyl stress was increased seven-fold, compromising electron transport efficiency and calcium retention.

Structured PICO

P
Population
8-10-week-old male and female mice
I
Intervention
Infusion of angiotensin-II (1.5 μg/g/day) and phenylephrine (50 μg/g/day) to induce experimental heart failure with preserved ejection fraction (HFpEF)
O
Outcome
Mitochondrial function, metabolic remodeling, and dicarbonyl/glycative stresssurrogate

In an experimental HFpEF model, metabolic remodeling drives dicarbonyl and glycative stress, which impairs mitochondrial function, suggesting mitochondrial dicarbonyl detoxification and anti-glycation strategies as potential therapeutic targets.

Cite This Study

Aryal et al. (2026) studied this question. Cardiac metabolic remodeling in experimental HFpEF drove a 7-fold increase in mitochondrial protein glycation and a ~20% reduction in mitochondrial calcium retention capacity.

synapsesocial.com/papers/69e9bb6285696592c86ed0b6https://doi.org/10.1152/ajpheart.00029.2026
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