Development, Validation, and Clinical Application of an HPLC-Based Method for Determining Adenosine Deaminase Activity in Adenosine Deaminase Deficiency-Severe Combined Immunodeficiency

Maria Petrik; Evgeny Litvin; Andrey Osipyants; Aleksandra Filkova; Yulia Rodina; Дмитрий Блинов; Nikolay Grachev; Anna Shcherbina

doi:10.1093/jalm/jfag037

March 12, 2026The Journal of Applied Laboratory Medicine

Development, Validation, and Clinical Application of an HPLC-Based Method for Determining Adenosine Deaminase Activity in Adenosine Deaminase Deficiency-Severe Combined Immunodeficiency

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Key Points

This research aims to develop and validate a method for measuring adenosine deaminase (ADA) activity in patients with ADA deficiency.
Developed a high-performance liquid chromatography (HPLC) method for ADA activity measurement.
Validated the method in dried blood spots (DBS).
Assessed applicability in diagnostic settings and scientific research.
The HPLC method provides precise and reliable measurements of ADA activity.
Successfully applicable for diagnosing adenosine deaminase deficiency.
Useful for monitoring patients and for research on purine metabolism.

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Implication

This validation study demonstrates a reliable method for determining ADA activity in patients, indicating its diagnostic utility.

Abstract

The developed method allows precise and reliable determination of ADA activity in DBS. It can be applied for the diagnosis and monitoring of patients with ADA deficiency, as well as in scientific studies related to purine metabolism.

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Cite This Study

Petrik et al. (2026) studied this question.

synapsesocial.com/papers/69e9bb6285696592c86ed14c https://doi.org/10.1093/jalm/jfag037

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Development, Validation, and Clinical Application of an HPLC-Based Method for Determining Adenosine Deaminase Activity in Adenosine Deaminase Deficiency-Severe Combined Immunodeficiency

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Development, Validation, and Clinical Application of an HPLC-Based Method for Determining Adenosine Deaminase Activity in Adenosine Deaminase Deficiency-Severe Combined Immunodeficiency

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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