Genome sequencing reveals antimicrobial resistance in Pseudomonas aeruginosa ST162 from bovine milk, suggesting zoonotic risks.
Key Points
This study aims to characterize the genomic features of Pseudomonas aeruginosa ST162 isolates from bovine milk in South Africa, focusing on antimicrobial resistance and virulence factors.
Isolates obtained from bovine milk (n=10) were analyzed using whole-genome sequencing, resistome profiling, and pangenome analysis.
Pangenomic analysis determined clade membership, revealing ST162 lineage shared with human strains from the US and Australia.
Phenotypic gene assessments included the presence of antimicrobial resistance genes and prophage identification.
Isolates exhibited a conserved resistome with specific antimicrobial resistance genes for β-lactams, aminoglycosides, tetracyclines, and sulfonamides.
Gene ppkA was absent in three isolates, while the pchH gene was variably present, indicating differences in virulence mechanisms.
Common prophages were found among isolates, emphasizing shared genomic features that may affect dissemination.