Establishing an air-liquid interface system for exposure of mouse laryngeal epithelial cells to cigarette smoke

Key Points

• This study aims to create a model that mimics real mouse laryngeal epithelial conditions to explore the effects of cigarette smoke.
• Developed an air-liquid interface system for mouse laryngeal epithelial cells.
• Characterized the physiological relevance of the model system.
• Assessed pathways involved in cigarette smoke-induced injury and repair.
• Established a model that mimics native laryngeal epithelium features.
• Demonstrated potential cellular and molecular pathways involved in epithelial injury from cigarette smoke.