A Gram-negative bacterium designated as strain TQ07 was isolated from a sawdust sample collected from a sawmill in the community of "El Salto" in the state of Durango, Mexico. This strain was previously reported for its ability to degrade chloranilic acid, a derivative of both biotic and abiotic degradation of pentachlorophenol. Genome sequencing was carried out using the Illumina NextSeq 500 platform. The draft genome of strain TQ07 was 5, 016, 215 bp in length, with 4, 504 protein-coding sequences and an average G+C content of 60. 3 mol%. A phylogenetic tree supports the assignment of strain TQ07 as a member of the genus Herbaspirillum. Comparison of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values revealed that strain TQ07 shared 98. 02% and 84. 3% identity, respectively, with the genome of H. lusitanum P6-12 (DSM-17154T^). These values indicate that strain TQ07 belongs to the same species as strain P6-12. Other bacterial strains were found to contain all or some orthologs of genes involved in chloranilic acid degradation. Additionally, strain TQ07 possesses genes encoding proteins for Cd 2+, Cu 2+, Cr 6+, and As 5+ tolerance. The draft genome sequence of H. lusitanum TQ07 has been deposited in the DDBJ/EMBL/GenBank databases under the accession number PRJNA484228. This genome sequence data provides insightful information for taxonomic characterization and further biotechnological exploitation of H. lusitanum TQ07 for the bioremediation of treated wood.
Salinas et al. (Wed,) studied this question.