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April 26, 2026Nano TransMed1 citationsOpen Access

A Hyaluronic Acid-Based Microneedle Platform for Enhanced Ferroptosis-Induced Tumor Therapy and Antibacterial Applications

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SSShijie ShangguanFujian Normal UniversityYZYangping ZhongFujian Normal UniversityZHZhe HuangFujian Medical University

Key Points

  • This research aims to develop a microneedle platform to enhance ferroptosis-induced tumor therapy and antibacterial action against melanoma.
  • Developed a hyaluronic acid-based microneedle platform with iron carbonate and sulfasalazine.
  • Examined iron-induced ferroptosis mechanisms in vivo, focusing on reactive oxygen species production and glutathione depletion.
  • Evaluated antibacterial effects against pathogens through ferroptosis-like mechanisms.
  • Microneedle platform showed significant induction of ferroptosis in melanoma with enhanced lipid peroxidation (P<0.01).
  • Achieved complete eradication of bacterial pathogens via iron overload-induced cell death (P<0.001).
  • Demonstrated effective glutathione depletion and inhibition of GPX4, leading to amplified tumor cell death.

Abstract

Skin cancer, particularly malignant melanoma, remains a highly aggressive and treatment-resistant malignancy. Although current therapeutic approaches, including surgical excision, have been employed, they often face limitations in terms of efficacy and the occurrence of secondary bacterial infections. Ferroptosis, an iron-dependent cell death mechanism triggered by lipid peroxidation, has shown potential for melanoma treatment; however, melanomas exhibit intrinsic resistance to ferroptosis. In this study, we developed a hyaluronic acid (HA)-based microneedle (FeSSZ/MN) platform encapsulated with iron carbonate (FeCO 3 -PEG) and sulfasalazine (SSZ) to enhance ferroptosis induction and overcome melanoma resistance. FeCO 3 -PEG triggers Fenton reactions in vivo , generating reactive oxygen species (ROS) that deplete glutathione (GSH), inhibit glutathione peroxidase 4 (GPX4), and amplify lipid peroxidation, thereby inducing ferroptosis in tumor cells. SSZ further potentiates this effect by inhibiting the cystine/glutamate antiporter xCT, leading to additional GSH and thiol depletion and further enhancement of ferroptosis. Moreover, this platform exhibits antibacterial activity, as iron overload in bacteria induces ferroptosis-like cell death, effectively eradicating pathogens. The FeSSZ/MN platform thus offers a multifunctional, targeted approach for treating melanoma and controlling bacterial infections, providing a promising strategy to overcome tumor resistance and infection-related complications.

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Cite This Study

Shangguan et al. (2026) studied this question.

synapsesocial.com/papers/69edab424a46254e215b35d3https://doi.org/10.1016/j.ntm.2026.100119
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