In recent years, bacteria-based self-healing has emerged as a promising bioengineering strategy to address the self-repair of cracks in cement-based materials, which represent one of the persistent durability challenges. This approach relies on microbiologically induced calcium carbonate (CaCO3) precipitation (MICP), in which metabolically active bacteria promote CaCO3 formation of crystals that can heal cracks and restore material integrity. This study compares the self-healing potential of a natural (N-) alkaline soil Bacillus licheniformis strain with a UV-strain (phenotypic mutant) generated through controlled UV exposure followed by adaptive evolution. Both strains were evaluated under conditions relevant to cementitious environments. The UV-strain exhibited enhanced ureolytic performance, reaching urease activity of 0.32 U/mg compared to 0.24 U/mg in the N-strain. This translated into improved biomineralization, with CaCO3 precipitation reaching 2.37 mg versus 2.23 mg/100 mL in the N-strain. Additionally, the UV-strain showed increased cell hydrophobicity and aggregation, indicating improved nucleation potential and surface-mediated mineral deposition. Multivariate analysis confirmed strong correlations between ureolytic metabolism, alkalization, and mineral formation, while artificial neural network (ANN) modeling (MLP 6-10-14) successfully predicted biomineralization-related parameters with high accuracy (R2 > 0.90 for urease activity, NH4+, ΔpH, and CaCO3). The results demonstrate that UV-induced phenotypic adaptation can enhance biomineralization efficiency with minor trade-offs in physiological robustness. For the first time, that controlled UV-induced phenotypic adaptation can be used as a targeted strategy to enhance biomineralization efficiency in B. licheniformis, while maintaining functional stability under cement-relevant conditions. These findings provide a novel framework for tailoring bacterial performance in self-healing systems for construction biotechnology.
Mejić et al. (Fri,) studied this question.