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April 26, 2026Reports — Medical Cases Images and Videos0 citationsOpen Access

Early-Onset Oral Tongue Squamous Cell Carcinoma in the Absence of Traditional Risk Factors: A Case Report with Whole-Exome Sequencing Analysis

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EAEvgeniy AleksievDKDarina Lyudmilova Kachakova-YordanovaVMVanyo Mitev

Key Points

  • Investigate the genetic factors in early-onset oral tongue squamous cell carcinoma in a non-smoking woman.
  • Case report detailing clinical presentation and treatment of a 33-year-old woman with oral tongue OSCC.
  • Whole-exome sequencing analysis conducted to identify genetic mutations and alterations.
  • Patient treated with surgical intervention followed by adjuvant radiotherapy.
  • Whole-exome sequencing identified a pathogenic truncating TP53 mutation.
  • Additional somatic alterations affecting multiple pathways were revealed.
  • Patient remains disease-free 15 months post-therapy.

Abstract

Oral squamous cell carcinoma (OSCC) typically develops in individuals with established risk factors such as tobacco and alcohol use, yet an increasing number of cases occur in young non-smoking, non-drinking (NSND) patients. We report a case of oral tongue OSCC in a 33-year-old woman who is a never-smoker and never-drinker without identifiable environmental or local risk factors. The patient underwent surgical treatment followed by adjuvant radiotherapy and remains disease-free 15 months after therapy. Whole-exome sequencing (WES) revealed a pathogenic truncating TP53 mutation together with additional somatic alterations affecting genes involved in DNA repair, hypoxia adaptation, mitochondrial function, and epigenetic regulation. The heterogeneous mutational profile suggests branched tumor evolution and the involvement of non-classical tumorigenic pathways. This report contributes to the growing evidence that OSCC in young NSND patients represents a biologically distinct subgroup and demonstrates the value of comprehensive genomic profiling for improving understanding of tumor heterogeneity and potential molecular drivers in the absence of traditional carcinogenic exposures.

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Cite This Study

Aleksiev et al. (2026) studied this question.

synapsesocial.com/papers/69edabdf4a46254e215b3b72https://doi.org/10.3390/reports9020130
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