Stereoselective hydrogen isotope exchange on nicotinamide cofactors through flavoenzyme microscopic reversibility

Christopher Otun; Michael Yuen; Harry Spacey; Carlo Bawn; Matthew Cliff; Francesco Falcioni; Ryan Ashley Ashley Bragg; Charles S. Elmore; Sam Hay; Jack; id_orcid 0000-0002-1590-8674 Rowbotham

doi:10.1039/d6sc01003b

January 1, 2026Chemical ScienceOpen Access

Stereoselective hydrogen isotope exchange on nicotinamide cofactors through flavoenzyme microscopic reversibility

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Key Points

The research aims to explore the ability of flavoenzymes to catalyze stereoselective hydrogen isotope exchange.
Panel of flavoenzymes assessed for hydrogen isotope exchange activity.
Use of D2O and reduced nicotinamide cofactors in experiments.
Evaluation of stereoselectivity during the exchange process.
Flavoenzymes effectively catalyzed hydrogen isotope exchange with significant stereoselectivity.
Demonstrated a redox-neutral pathway for isotope incorporation.
Results indicate potential for expanded applications in biochemical synthesis.

Implication

Demonstrates hydrogen isotope exchange in nicotinamide cofactors, suggesting new pathways for biochemical reactions.

Abstract

We describe a panel of flavoenzymes with the ability to catalyse stereoselective hydrogen isotope exchange (HIE) between 2 H 2 O (D 2 O) and reduced nicotinamide cofactors, enabling a simple and redox-neutral route to...

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Cite This Study

Otun et al. (2026) studied this question.

synapsesocial.com/papers/69edac9b4a46254e215b44b0 https://doi.org/10.1039/d6sc01003b

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Stereoselective hydrogen isotope exchange on nicotinamide cofactors through flavoenzyme microscopic reversibility

Key Points

Implication

Abstract

Discussion

Cite This Study

Stereoselective hydrogen isotope exchange on nicotinamide cofactors through flavoenzyme microscopic reversibility

Key Points

Implication

Abstract

Discussion

Cite This Study

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