Cytoskeletal Constraints Shape Astrocyte Network Dynamics · Cytoszkieletowa infrastruktura astrocytów ogranicza dynamikę wielkoskalowej sygnalizacji glejowej

Bilingual Preprint (EN ∥ PL) Astrocytes form a brain-wide, gap-junction-coupled network that supports spatially extended calcium signaling and modulates neuronal activity. We propose that astrocytic cytoskeletal architecture — particularly microtubule-dependent transport and morphology — defines a set of biophysical constraints that shape the accessible space of glial signaling dynamics. Three experimentally grounded mechanisms are synthesized: (i) cytoskeletal maintenance of astrocyte morphology and spatial coverage; (ii) microtubule-based transport of organelles and signaling machinery required for calcium-wave propagation; and (iii) cytoskeleton-dependent trafficking and stabilization of connexin-based gap junctions. The framework yields testable predictions linking cytoskeletal integrity to emergent network behavior, is supported by a minimal reaction–diffusion model, and provides a multiscale bridge between intracellular organization and large-scale glial dynamics. v6.0 — Fig. 4 re-labeled as prediction figure (not experimental data); caption explicitly states values illustrate predicted direction and magnitude. Added mechanistic literature references (Basu 2017, Chandrasekhar & Bera 2017, Scemes, Blomstrand 1999).