The storage time dependence on protective effect by pressurized dissolution of argon gas in cold storage on a rat heart striated myocytes monolayer were investigated. The cell monolayers incubated in a culture dish for 24 h were prepared as test samples. The samples were pressurized in 0 and 0.8 MPa in argon gas, and then preserved at 6°C for 0 to 72 h. The pressure-dependent cell viability was evaluated using water-soluble tetrazolium salts assay. The results showed that protective effect in after 24 h was low without pressure, but it was high with pressure. The protective effects in storage time of 48 h and 72 h were low even with pressurized dissolution of argon gas, but they were slightly higher than that without it.
Mimura et al. (2025) studied this question.