Zona pellucida proteins Piopio and Dumpy are essential for the apical extracellular matrix assembly and survival of Locusta migratoria

Key Points

• The study aims to investigate the roles of LmPio and LmDpy proteins in extracellular matrix assembly and cuticle integrity in Locusta migratoria.
• Examined LmPio and LmDpy gene function using knockdown techniques
• Assessed cuticle integrity and survival in Locusta migratoria through experimental interventions
• Knockdown of LmPio or LmDpy led to high mortality rates among Locusta migratoria
• The study demonstrated the essentiality of ZP proteins for apical extracellular matrix assembly
• Findings suggest potential for RNAi-based pest control targeting these proteins