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Rational design enhances biocatalysis and yields 208 g/L of α-GG from sucrose and glycerol, highlighting enzyme engineering's potential.
-α-GG) production from 1.8 M sucrose and 2.16 M glycerol (a 208 g/L increase over the wild type) at 45 °C. This study highlights the industrial merits of rational integrated computational approaches, enabling efficient enzyme engineering for superior biocatalysis in high-viscosity systems.
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Xu et al. (2026) studied this question.
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