An Ene Reductase, KlebER1, for the Production of Dihydrocarvone: Identification, Characterization, Engineering, and Application

for dihydrocarvone production. This biocatalytic system achieved a dihydrocarvone concentration of 5.88 mmol/L and a conversion rate of 99%, representing a significant improvement compared to the single use of A303N mutant. This study lays the foundation for investigating the function and modification of ERs and promoting the efficient biosynthesis of dihydrocarvone.