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April 30, 2026Genes to CellsOpen Access

Histone Density and Dynamics Shape Mitotic Chromatid Architecture in Xenopus Egg Extracts

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Authors

KSKeishi ShintomiKOKeita OhsumiMIM. Iwabuchi

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Overview

Modified assay demonstrates chromatin organization and condensins shape mitotic chromatid architecture.

Key Points

  • To investigate how histone density and dynamics influence mitotic chromatid architecture.
  • Utilized a modified cell-free assay with erythrocyte nuclei in Xenopus mitotic egg extracts.
  • Separated chromatid formation from nucleosome assembly by using dense nucleosome arrays.
  • Examined effects of linker histone H1.8, histone chaperone Nap1, and chromatin remodeler ISWI on chromatid structure.
  • Depletion of linker histone H1.8 resulted in thinner chromatids and enhanced individualization.
  • Excess H1.8 loading promoted chromatid clustering.
  • Clustering was also observed with depletions of Nap1 and ISWI, highlighting their roles in chromatid dynamics.

Cite This Study

Shintomi et al. (2026) studied this question.

synapsesocial.com/papers/69f2a42a8c0f03fd677633bfhttps://doi.org/10.1111/gtc.70113
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Single-nucleosome imaging unveils that condensins and nucleosome–nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes2024 · 16 citations
  2. 2High resolution maps of chromatin reorganization through mouse meiosis reveal novel features of the 3D meiotic structure2024 · 9 citations
  3. 3Spindle morphology changes between meiosis and mitosis driven by CK2 regulation of the Ran pathway2024 · 1 citations
  4. 4Atomic force microscopy identifies filamentous structures comprising mitotic chromosomes2026
  5. 5Hbo1 and Msl complexes preserve differential compaction and H3K27me3 marking of active and inactive X chromosomes during mitosis2024 · 1 citations