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April 30, 2026Free Radical Research0 citations

Oxidation of fluorogenic probes detecting reactive oxygen species in the extracellular medium

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MRMałgorzata RakRzeszów University of TechnologyGBGrzegorz BartoszRzeszów University of TechnologyISIzabela Sadowska-BartoszRzeszów University of Technology

Key Points

  • This research aims to compare the oxidation rates of fluorogenic probes in cell culture and cell-free media.
  • Compared oxidation rates of different fluorogenic probes in both cell culture and cell-free media.
  • Analyses focused on the effects of cell-free medium components on probe properties.
  • Higher oxidation rates observed in cell-free medium across all tested probes.
  • Results indicate that autoxidation of redox-active components significantly affects the medium's properties.

Abstract

DCF-DA) in cell culture and cell-free media was compared. Comparable or higher oxidation rates in cell-free medium were found for all probes. These results point to autoxidation of redox-active components of the medium as the main cause of changes in the properties of cell culture media and suggest that the intracellular oxidation of the probes may also be partly due to their autoxidation.

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Cite This Study

Rak et al. (2026) studied this question.

synapsesocial.com/papers/69f2f0e31e5f7920c6386ec4https://doi.org/10.1080/10715762.2026.2665470
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