Oxidation of fluorogenic probes detecting reactive oxygen species in the extracellular medium

Key Points

• This research aims to compare the oxidation rates of fluorogenic probes in cell culture and cell-free media.
• Compared oxidation rates of different fluorogenic probes in both cell culture and cell-free media.
• Analyses focused on the effects of cell-free medium components on probe properties.
• Higher oxidation rates observed in cell-free medium across all tested probes.
• Results indicate that autoxidation of redox-active components significantly affects the medium's properties.