Stability-Indicating Rp-HPLC Method Development and Validation for the Simultaneous Estimation of Luliconazole and Salicylic Acid in Its Topical Dosage Form

A simple, rapid, precise, and stability-indicating RP-HPLC method was developed and validated for the simultaneous estimation of Luliconazole and Salicylic Acid in pharmaceutical dosage form. Separation was achieved on a YMC Pack ODS-A column (150 mm × 4.6 mm, 5 µm) using Buffer: Methanol (30:70 % v/v) as mobile phase at a flow rate of 1.0 mL/min, with detection at 226 nm. The method showed excellent linearity in the range of 10–30 µg/mL for Luliconazole and 30–90 µg/mL for Salicylic Acid (R² > 0.998). Accuracy studies showed recoveries of 98% and 99.6%, respectively. LOD and LOQ values indicated good sensitivity. Forced degradation studies under various stress conditions confirmed the stability-indicating nature of the method, with clear separation of degradation products. The method is suitable for routine quality control analysis.