Traceless protein semisynthesis in cells using the promiscuous ultra-fast split intein NrdJ-1

Xuanjia Ye; Joshua Sokol; Christian Kofoed; Anushka Dheer; Juner Zhang; Tom W. Muir

doi:10.1039/d6sc01808d

January 1, 2026Chemical ScienceOpen Access

Traceless protein semisynthesis in cells using the promiscuous ultra-fast split intein NrdJ-1

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Key Points

This research aims to explore the efficiency and versatility of the serine-based split intein NrdJ-1 for protein semisynthesis in live cells.
Utilized the serine-based split intein NrdJ-1 for generating semisynthetic proteins in cellular context.
Evaluated the efficiency of post-translational modifications on chromatin in living cells.
NrdJ-1 facilitated the effective and traceless modification of chromatin proteins.
The process showed high efficiency, enabling rapid generation of modified proteins without detectable residues.

Implication

Demonstrates traceless semisynthesis of modified chromatin in living cells, indicating potential for advancing genetic research.

Abstract

Serine-based split intein NrdJ-1 exhibits context promiscuity and high efficiency, enabling traceless generation of semisynthetic, post-translationally modified chromatin in living cells.

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Cite This Study

Ye et al. (2026) studied this question.

synapsesocial.com/papers/69f442fc967e944ac55666c0 https://doi.org/10.1039/d6sc01808d

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Traceless protein semisynthesis in cells using the promiscuous ultra-fast split intein NrdJ-1

Key Points

Implication

Abstract

Discussion

Cite This Study

Traceless protein semisynthesis in cells using the promiscuous ultra-fast split intein NrdJ-1

Key Points

Implication

Abstract

Discussion

Cite This Study

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