Inhibition of AP-1 Reduces CD46-mediated Invasion of Bladder and Colon Cancer Cells.

BACKGROUND/AIM: the inactivation of MMP9. MATERIALS AND METHODS: Both bladder and colon cancer cells were used for western blot analysis and reporter transcription assays. AP-1 inhibitors used are AP-1 decoy oligonucleotide (AP1-ODN) and AP-1 specific inhibitor (SR11302). To test AP-1-mediated inhibition of cell migration and invasion, both wound healing scratch assay and transwell chamber assays with or without gelatin coating were utilized. CONCLUSION: Targeting AP-1 can effectively suppress CD46-mediated invasion and metastasis in bladder and colon cancers, in which CD46 is generally overexpressed.