Characterization of antibodies for ARID1B enhances selection for specific applications in research, indicating potential performance issues.
<ns3:p>ARID1B encodes a core subunit of the SWI/SNF (BAF) ATP-dependent chromatin-remodeling complex and plays a critical role in transcriptional regulation, cell differentiation, and development. Here we have characterized seven ARID1B commercial antibodies for western blot and immunoprecipitation by using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.</ns3:p>
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Moleón et al. (2026) studied this question.
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