Reconstruction of Tissue-Engineered Conjunctiva Using Human Amniotic Epithelial Cells and Decellularized Porcine Conjunctiva Matrix With RADA16-I Peptide Hydrogel in a Perfusion Culture System.

), while maintaining stemness marker ABCG2 expression. After 3 days, the constructs showed significant upregulation of conjunctival epithelial markers (CK4, CK13, Muc5AC) at both protein and transcriptional levels, indicating successful transdifferentiation of hAECs toward a conjunctival epithelial phenotype. This integrated approach generates a functional tissue-engineered conjunctiva that promotes hAEC proliferation, stemness maintenance, and differentiation into conjunctival epithelial cells, offering a promising alternative for ocular surface reconstruction.