Sex-dependent modulation of CGRPergic neurovascular activity by 5-CT in rats

Figures

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  FIGURE 1. Study design showing the number of animals used in the two main sets and their subdivisions (n = 5 each). Vasodepressor responses were obtained either by electrical stimulation of the perivascular sensory outflow (set 1; groups 1–2: male rats; groups 3–4: female rats) or i.v. bolus administration of exogenous α-CGRP (set 2: female rats). D-R curve: dose-response curve; EtOH: ethanol; S-R curve: (electrical) stimulus-response curve.

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  FIGURE 2. Original experimental tracings showing mean blood pressure (MBP; mmHg), heart rate (HR; beats/min, bpm) and vasodepressor responses induced by (A) electrical stimulation of the perivascular CGRPergic outflow or (B) i.v. bolus administration of α-CGRP, during continuous i.v. infusion of saline (1 mL/h) in female pithed rats.

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  FIGURE 3. Effect of continuous i.v. perfusion of saline (1 mL/h; control), 5−CT (0.001 μg/kg/min), CP-93,129 (20 μg/kg/min), LY344864 (20 μg/kg/min), 5−CT (0.001 μg/kg/min) in the presence of SB699551 (1 mg/kg i.v.) or AS-19 (20 μg/kg/min) (n = 5 each) on the vasodepressor responses induced by electrical stimulation of the CGRPergic outflow in male rats. Data were analysed by two-way ANOVA followed by Dunnett’s post hoc test. All values are expressed as mean ± SEM. *P < 0.05 vs. saline. ΔMBP: changes in mean blood pressure.

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  FIGURE 4. Effect of continuous i.v. perfusion of saline (1 mL/h; control), ethanol 5% (EtOH, 1 mL/h), 5−CT (0.001 μg/kg/min) or AS-19 (20 μg/kg/min) (n = 5 each) on the vasodepressor responses induced by electrical stimulation of the CGRPergic outflow in female rats. Data were analysed by two-way ANOVA followed by Dunnett’s post hoc test. All values are expressed as mean ± SEM. *P < 0.05 vs. saline. ΔMBP: changes in mean blood pressure.

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  FIGURE 5. Effect of continuous i.v. perfusion of saline (1 mL/h; control), 8-OH-DPAT, sumatriptan or LY344864 (20 μg/kg/min and n = 5 each) on the vasodepressor responses induced by electrical stimulation of the CGRPergic outflow in female rats. Data were analysed by two-way ANOVA followed by Dunnett’s post hoc test. All values are expressed as mean ± SEM. *P < 0.05 vs. saline. ΔMBP: changes in mean blood pressure.

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  FIGURE 6. Effect of continuous i.v. perfusion of 5−CT (0.001 μg/kg/min) alone or in the presence of SB699551 (1 mg/kg i.v.) or a cocktail of GR127935 (310 μg/kg i.v.) + SB258719 (1 mg/kg i.v.) on the vasodepressor responses induced by electrical stimulation of the CGRPergic outflow in female rats. Data were analysed by two-way ANOVA followed by Dunnett’s post hoc test. All values are expressed as mean ± SEM. *P < 0.05 vs. saline; #P < 0.05 vs. 5−CT alone. ΔMBP: changes in mean blood pressure.