Activity dynamics in the NPY neuronal signaling of mPFC in response to an air puff.

signal detected from mPFC→BLA projections in response to the air puff stress. Viral tracing demonstrated that, while NPY neurons in the mPFC receive monosynaptic input from many brain regions, their axonal output is restricted to layers of the mPFC, with one of the targets being local PVs. These results demonstrate that ongoing inhibition of PV activity in the mPFC by NPY via Y1r influences anxiety-like activity in mice, likely through strengthening mPFC output to the BLA. Subjecting animals to an aversive air puff acute stress disrupts this circuitry, providing further and new support for a role of NPY in the mPFC and the modulation of stress-related behaviors.