Answers are grounded in this paper and cite their sources.
Demonstrates effective fish species detection in wetland ecosystems, highlighting eDNA protocol improvements.
, half‑life = 6.8 days), falling to 2.18 ± 0.42 by Day 20. Longmire's buffer maintained statistically stable yields across storage (p > 0.05 for all pairwise comparisons), with no significant degradation. Ward barcode consistently amplified Longmire's preserved samples at 50 to 400 ng/μL; PBS samples showed no amplification. Ac12S showed limited amplification in both buffers. Application of the modified protocol in Harike wetland field samples confirmed its sensitivity and practical utility, detecting fish eDNA of Cyprinus carpio, Pethia conchonius, and Lepidocephalichthys thermalis. The findings suggest that buffer selection and standardized extraction are critical for reliable eDNA-based diversity surveys. The optimized workflow enhances flexibility for sampling in resource-limited areas, facilitating non-invasive surveys in protected wetlands.
No takes yet. Share an insight, caveat, or question.
Saikia et al. (2026) studied this question.
Synapse has enriched 8 closely related papers on similar clinical questions. Consider them for comparative context: