mediated 3,3',5,5'-tetramethylbenzidine (TMB) oxidation, producing a blue color readout. The catalytic mechanism was validated through steady-state kinetics and radical scavenging studies. The sensor achieves a wide linear range from 0.1 to 10 U/L, a low limit of detection (LOD) of 0.043 U/L, and strong anti-interference performance. Coupled with smartphone-based color analysis, this method enables rapid, portable ACP quantification in human plasma with relative standard deviation below 5%. This work establishes a sensitive point-of-care testing (POCT) strategy for ACP and provides a generalized framework for stabilized in-situ nanozyme synthesis, mechanistic investigation, and portable detection in next-generation biosensor design.
Lian et al. (Fri,) studied this question.