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May 1, 2026

Single-cell proteomics reveals cytoplasmic defects in Patl2-deficient oocytes rescued by spindle transfer.

A Cardona Barberán E Araftpoor A Christodoulaki M Fakhar-I-Adil J Goethals A Rybouchkin C Arnoult A Boel M Bühler K C Pavani D Stoop K Gevaert F Vanden Meerschaut B Heindryckx

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Key Points

This research aims to identify proteins associated with reduced developmental competence in Patl2-/- oocytes and evaluate spindle transfer as a potential rescue technique.
Utilized single-cell proteomics to analyze in vivo MII oocytes from Patl2-/- and Patl2+/+ females.
Conducted spindle transfer to Patl2+/+ cytoplasm followed by activation using SrCl2 exposure.
Performed statistical analysis on differentially expressed proteins post-proteomics.
Patl2-/- females had a lower MII rate (79.63%) compared to Patl2+/+ (89.39%, P = 0.0123).
Post-ICSI, Patl2-/- oocytes showed reduced activation rates (31.71%) and blastocyst rates (7.69%) versus Patl2+/+ oocytes (76.74% and 42.42%, respectively) with significant p-values.
Spindle transfer significantly improved activation rates (100%) and blastocyst rates (75%) in Patl2-/- oocytes, demonstrating effective rescue.

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Implication

Reveals abnormal protein profiles in Patl2-/- oocytes, suggesting spindle transfer as a rescue option for infertility.

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Cite This Study

Barberán et al. (2026) studied this question.

synapsesocial.com/papers/69f6e5308071d4f1bdfc5f66 https://doi.org/10.1093/humrep/deag073

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Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Identification of two novel PATL2 variants and transcriptome sequencing reveals their role in oocyte maturation.Original Research · 2026
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