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The study investigates how nucleobases coordinate with magnesium ions to facilitate RNA cleavage mechanisms.

May 3, 20260 citations

Nucleobase Coordination With Mg2 + Facilitates RNA Cleavage via Internal Transesterification.

KLKuan LuNankai University SHShiqing HuangRenmin University of China ZYZhilei YuNankai University

Key Points

The study investigates how nucleobases coordinate with magnesium ions to facilitate RNA cleavage mechanisms.
Analysis of rA cleavage kinetics at pyrimidine sites
Assessment of activation barriers for RNA cleavage
Comparison of spontaneous degradation and catalyzed cleavage methods
Lower activation barriers for RNA cleavage were observed when nucleobases coordinated with Mg2+.
Faster cleavage kinetics were demonstrated at pyrimidine sites, rationalizing preferential alkali-catalyzed cleavage.
New insights into the mechanistic role of nucleobases in RNA backbone scission were provided.

Abstract

of rA, leading to lower activation barriers and faster cleavage kinetics, thereby rationalizing the preferential alkali-catalyzed RNA cleavage at pyrimidine sites. These findings uncover a previously unrecognized mechanistic role of nucleobases in RNA backbone scission and provide new insights into spontaneous RNA degradation and RNase- and ribozyme-catalyzed RNA cleavage.

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