A fluorescence-activated droplet sorting assay for ultra-high-throughput screening of PET hydrolases based on a pH indicator.

Maxine Yew; Haizhen Bi; Dingjie Wang; Rui Qi; Huiling Yuan; Huanhuan Zhai; Gen Li; Qinhong Wang; Leilei Zhu

doi:10.1039/d6cb00122j

April 29, 2026

A fluorescence-activated droplet sorting assay for ultra-high-throughput screening of PET hydrolases based on a pH indicator.

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Key Points

This research aims to develop a pH-based fluorescence-activated droplet sorting assay for screening PET hydrolases efficiently.
Integration of a pH indicator in the fluorescence-activated droplet sorting assay.
Assessment of the assay's performance in ultra-high-throughput conditions.
Evaluation of PET hydrolase activity through fluorescence detection.
The pH-based assay successfully identifies functional PET hydrolases with high throughput.
Efficiency metrics indicate superior performance compared to conventional screening methods.

Implication

Demonstrates the efficiency of a pH-based assay for PET hydrolases, suggesting improved screening techniques.

Abstract

PETase, respectively. The successful integration of the pH-based assay with FADS highlights its versatility and efficiency for ultra-high-throughput screening of PET hydrolases.

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Cite This Study

Yew et al. (2026) studied this question.

synapsesocial.com/papers/69f6e5f38071d4f1bdfc694c https://doi.org/10.1039/d6cb00122j

View Full Paper

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A fluorescence-activated droplet sorting assay for ultra-high-throughput screening of PET hydrolases based on a pH indicator.

Key Points

Implication

Abstract

Discussion

Cite This Study

A fluorescence-activated droplet sorting assay for ultra-high-throughput screening of PET hydrolases based on a pH indicator.

Key Points

Implication

Abstract

Discussion

Cite This Study

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