Structure-Guided Computational Design and Electrochemical Validation of a DNA Aptamer for Impedimetric Detection of Shiga-like Toxin 2.

Key Points

• This study aims to develop a cost-effective and efficient method for synthesizing DNA aptamers for detecting shiga-like toxin 2.
• Utilized structure-guided computational design to create DNA aptamers.
• Conducted electrochemical validation of aptamer functionality.
• Compared new synthesis protocol to traditional SELEX methods.
• Successfully designed DNA aptamers that specifically bind to shiga-like toxin 2.
• Achieved detectable limits for impedimetric sensing with high specificity.
• Demonstrated faster synthesis and improved efficiency over traditional methods.