Metabolome and transcriptome analyses reveal metabolic differences and identify candidate regulatory factors among three habitat types of Gentiana scabra Bunge
Comparative analysis reveals metabolic differences in Gentiana scabra among diverse habitats, suggesting pathways for quality enhancement.
Key Points
The study aims to explore the metabolic differences of Gentiana scabra among various habitats and identify regulatory factors associated with these differences.
Conducted UPLC-MS/MS-based metabolomic analysis and RNA-seq-based transcriptomic analysis.
Identified 1824 metabolites and 93076 Unigenes across three habitat types: wild shrublands, wild meadows, and cultivated farmlands.
Analyzed differential metabolites and differentially expressed genes between pairs of habitats.
Identified 309 differential metabolites and 645 DEGs between wild meadows and wild shrublands.
Found 348 DMs and 605 DEGs between cultivated farmland and wild meadows.
Recognized 510 DMs and 917 DEGs between cultivated farmland and wild shrublands, indicating significant metabolic variation.