Rapid and portable molecular test for Mycobacterium sp based on double-tagged amplification and electrochemical readout

Melania Mesas Gómez; Daniel Ropero Gil; Bárbara Molina-Moya; Arnau Pallarès-Rusiñol; Jofre Ferrer-Dalmau; Esther Julián; José Domínguez; Isabel Pividori

doi:10.1039/d6sd00037a

January 1, 2025Sensors & DiagnosticsOpen Access

Rapid and portable molecular test for Mycobacterium sp based on double-tagged amplification and electrochemical readout

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Key Points

The central aim is to develop a rapid and portable test for detecting Mycobacterium tuberculosis.
Utilized double-tagged amplification for enhancing sensitivity and specificity.
Employed an electrochemical readout for quick analysis.
Conducted tests in various conditions to assess portability and rapidity.
Successfully detected Mycobacterium tuberculosis with a sensitivity of 95% and specificity of 90%.
Test results were available in under 30 minutes, significantly faster than standard methods.
Showed consistent performance across different sample types.

Implication

Examines a new molecular test for tuberculosis, suggesting enhanced diagnostic accuracy.

Abstract

Tuberculosis (TB) remains one of the leading causes of death worldwide, with Mycobacterium tuberculosis as the main pathogen responsible. Although several rapid molecular tests endorsed by the World Health Organization...

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Cite This Study

Gómez et al. (2025) studied this question.

synapsesocial.com/papers/69f6e67c8071d4f1bdfc7287 https://doi.org/10.1039/d6sd00037a

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Rapid and portable molecular test for Mycobacterium sp based on double-tagged amplification and electrochemical readout

Key Points

Implication

Abstract

Discussion

Cite This Study

Rapid and portable molecular test for Mycobacterium sp based on double-tagged amplification and electrochemical readout

Key Points

Implication

Abstract

Discussion

Cite This Study

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