Anhydrotetracycline-Inducible Metabolic Flux Adaptation for 3-Hydroxypropionic Acid Production in Pichia pastoris.

Key Points

• This research aims to enhance the production of 3-hydroxypropionic acid using an inducible metabolic flux adaptation system in Pichia pastoris.
• Applied anhydrotetracycline-inducible system to regulate gene expression in Pichia pastoris.
• Designed and tested metabolic pathways for optimized production of 3-hydroxypropionic acid.
• Achieved a significant increase in 3-hydroxypropionic acid production compared to baseline levels.
• Demonstrated improved metabolic flux with the inducible system leading to enhanced product yield.