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May 3, 2026Frontiers in Pediatrics0 citationsOpen Access

Sublingual methylcobalamin treatment in infants with prolonged jaundice due to vitamin B12 deficiency

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SASultan AydinAÖAyşe ÖzHTHayrullah Türkmen

Key Points

  • This research investigates the effects of sublingual methylcobalamin treatment on prolonged jaundice caused by vitamin B12 deficiency in infants.
  • Conducted as a descriptive cross-sectional study in a pediatric hematology department.
  • Included 103 infants with prolonged jaundice due to vitamin B12 deficiency, divided into two treatment groups: sublingual methylcobalamin and intramuscular cyanocobalamin.
  • Measured serum vitamin B12 levels before treatment and at follow-ups of 1.5 and 3 months.
  • Group 1 (sublingual methylcobalamin) showed mean serum vitamin B12 levels increased from 138.82 ng/L to 656.86 ng/L at 1.5 months (p<0.001).
  • Group 2 (intramuscular cyanocobalamin) had mean vitamin B12 levels rise from 165.71 ng/L to 502.9 ng/L at 1.5 months (p<0.001).
  • Significant reduction in prolonged jaundice observed in infants treated with methylcobalamin compared to cyanocobalamin.

Abstract

Objectives Ineffective erythropoiesis resulting from vitamin B12 deficiency leads to hemolysis. The breakdown of erythrocytes increases plasma bilirubin levels, which can cause jaundice in newborns. Methods This descriptive cross-sectional study was conducted at the Department of Pediatric Hematology, Antalya Training and Research Hospital. In total, 103 infants with prolonged neonatal jaundice due to vitamin B12 deficiency, defined as a serum level below 250 ng/L, were included. Participants were divided into two groups based on treatment type: sublingual methylcobalamin (Group 1, n = 72) and intramuscular cyanocobalamin (Group 2, n = 31). Results The mean gestational ages were 38.36 ± 1.4 weeks in Group 1 and 38.87 ± 0.81 weeks in Group 2. The mean ages at diagnosis were 35.74 ± 13.17 days and 35.9 ± 11.73 days, respectively. The female-to-male ratios were 30:42 in Group 1 and 16:15 in Group 2. Mean serum vitamin B12 levels in Group 1 were 138.82 ± 50.09 ng/L before treatment, 656.86 ± 333.16 ng/L at 1.5 months, and 394.08 ± 169.94 ng/L at 3 months following sublingual methylcobalamin therapy. In Group 2, the mean levels were 165.71 ± 56.07 ng/L before treatment, 502.9 ± 196.07 ng/L at 1.5 months, and 345.77 ± 115.42 ng/L at 3 months after intramuscular cyanocobalamin administration. Conclusion Vitamin B12 deficiency may be associated with prolonged jaundice in neonates. Early diagnosis of vitamin B12 deficiency enables timely initiation of sublingual methylcobalamin, a non-invasive and effective treatment option.

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Cite This Study

Aydin et al. (2026) studied this question.

synapsesocial.com/papers/69f6e6ab8071d4f1bdfc7691https://doi.org/10.3389/fped.2026.1822723
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