Development and Validation of a Sensitive UHPLC-MS/MS Method for the Simultaneous Determination of 19 Bile Acids in Liver and Feces: Application to Metabolic Dysfunction-Associated Steatotic Liver Disease Mice.

≥ 0.991), extraction recovery (82%-112%), limits of detection (1-2 ng/mL) and low limits of quantification (2-5 ng/mL). Then, the method was applied to the detection of BAs in liver and feces of metabolic dysfunction-associated steatotic liver disease mice, and the correlations between BA levels and the mRNA expression levels of BA metabolic enzymes, transporters, and receptors were analyzed. This study provides a direct and reliable determination method for the in-depth investigation of the enterohepatic circulation of BAs.