A novel peroxidase-mimicking Cu, S co-doped carbon dot nanozyme for dual-mode lactate biosensing in serum and sweat

Key Points

• This research aims to develop a novel nanozyme for effective lactate biosensing.
• Utilized Cu, S co-doped carbon dots as a nanozyme mimicking peroxidase activity.
• Evaluated lactate levels in serum and sweat using the developed biosensor prototype.
• Conducted comparative analyses to assess sensitivity and specificity for lactate detection.
• Demonstrated enhanced lactate detection with sensitivity and specificity in both serum and sweat samples.
• Achieved a detection limit of X μM with significant accuracy (p<0.001).
• Validated the nanozyme performance through clinical testing against standard lactate monitoring methods.