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May 2, 2026

Designing strategies for high-level production of peptide-N-glycosidase F (PNGase F) from Flavobacterium meningosepticum using high cell density fed-batch culture of E. coli.

Shilpa Mohanty Babbal Shivani Chauhan Mohini Talwar Shubham Sharma Priya Maravi Yogender Pal Khasa

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Key Points

The aim is to explore different E. coli hosts to enhance the production of PNGase F in a cost-effective manner.
Utilized high cell density fed-batch culture techniques.
Investigated multiple E. coli strains as expression hosts for PNGase F production.
Identified promising E. coli strains that significantly improve PNGase F yield.
Demonstrated potential cost reductions in the production process.

Implication

Investigates alternative E. coli hosts for cost-effective PNGase F production, suggesting new strategies.

Abstract

of 176. Therefore, this study investigates the potential of alternative E. coli hosts as cost-effective production platforms for PNGase F.

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Cite This Study

Mohanty et al. (2026) studied this question.

synapsesocial.com/papers/69f9890415588823dae17eec https://doi.org/10.1007/s00449-026-03337-3

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