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May 6, 20260 citations

Enhancing immunogenicity of rabies virus glycoprotein with dendritic cell-targeting peptide

Enhanced immunogenicity of the rabies virus glycoprotein fused to a dendritic cell-targeting peptide.

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Authors

ABAtousa BakhsheshiFSFattah SotoodehnejadnematalahiAGAlireza Gholami

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Overview

This research demonstrates improved immunogenicity in eukaryotic cells through rabies vaccine development using targeted peptides.

Key Points

  • To enhance the immunogenicity of rabies virus glycoprotein by fusing it with a dendritic cell-targeting peptide.
  • Cloning rabies virus glycoprotein in pCDNA3.1+ vector
  • Fusing dendritic cell-targeting peptide to the glycoprotein
  • Transfecting the recombinant plasmid into eukaryotic BHK-21 cells using lipofection
  • Confirming protein expression through SDS-PAGE and western blotting
  • Purifying the His-tagged recombinant protein using Ni-NTA resin
  • Successful expression of RVG-DCP fusion protein in eukaryotic cells
  • Enhanced immune response indicated by increased IgG levels
  • Potential applications for rabies vaccine development noted
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Cite This Study

Bakhsheshi et al. (2026) studied this question.

synapsesocial.com/papers/69fa980604f884e66b531c8fhttps://doi.org/10.1038/s41598-026-51181-y
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