A biosensor containing Ag nanoparticles in ionic liquid (IL) 1‐butyl‐3‐methylimidazolium bis(trifluoromethylsulfonyl)imide (BMI.Tf 2 N) and laccase (Lac) immobilized on β ‐cyclodextrin modified with epichlorohydrin ( β ‐CDEpi) was developed for quercetin determination. Lac catalyses the oxidation of quercetin to quinone, which is then reduced on the biosensor surface and the resulting current was investigated by square‐wave voltammetry. The β ‐CDEpi support was analyzed by scanning electron microscopy and Ag‐BMI.Tf 2 N by transmission electron microscopy and X‐ray diffraction. The linear range (0.499–7.407 μM) and low detection limit (0.037±0.004 μM) show that the proposed biosensor is suitable for quercetin determination in real samples.
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Franzoi et al. (2010) studied this question.
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