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January 1, 2026Open Access

Evaluation of single-stranded DNA knock-in and base editing in combination with sleeping beauty transposon for a non-viral allogeneic 19BBz CAR-T generation

Eduardo Correia Paulo Schuroff Davi Torres Luiza Abdo Emmanuel Aragão João Steimbach Paula Alves Bernardo Vasconcelos Ricardo Weinlich Martín Bonamino

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Key Points

The study aims to evaluate the effectiveness of single-stranded DNA knock-in and base editing in generating CAR-T cells.
Evaluated the combination of base editing and sleeping beauty transposon on CAR-T generation
Utilized non-viral methods for allogeneic CAR-T development
Demonstrated successful generation of 19BBz CAR-T cells using the evaluated techniques
Showed enhanced transfection efficiency with the combined approach

Implication

Evaluates DNA knock-in and base editing for CAR-T development, suggesting non-viral methods are effective.

Abstract

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Cite This Study

Correia et al. (2026) studied this question.

synapsesocial.com/papers/69fd7cd4bfa21ec5bbf05c32 https://doi.org/10.35259/isi.2026_76393

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