Hyperspectral tomographic diffractive microscopy: Development and applications

Tomographic Diffractive Microscopy (TDM) provides label‐free three‐dimensional imaging of transparent samples with resolution surpassing confocal limits. At IRIMAS, successive instrumental developments since 2009 have enhanced TDM capabilities through transmission, reflection, isotropic, polarisation‐sensitive, and dual‐view configurations. However, conventional single‐wavelength TDM cannot distinguish chemically distinct structures with similar optical properties at the measurement wavelength. This article presents the development of hyperspectral TDM, which exploits wavelength‐dependent optical properties to provide chemical specificity through 3D spectroscopic characterisation. We describe the physical principles underlying TDM reconstruction, detail our integration of a supercontinuum source for multi‐wavelength capability, and outline applications in biomedical and material imaging. Our structured development roadmap encompasses instrumentation, calibration, hyperspectral synthesis, and AI‐enhanced reconstruction.