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May 8, 2026European Stroke JournalOpen Access

Point-of-care CYP2C19 genotyping detects loss-of-function variants in ~26% of stroke patients but suffers frequent test failures.

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Why the study?

Recent guidelines recommend pharmacogenetic testing before clopidogrel prescription to identify CYP2C19 loss-of-function variants associated with reduced drug efficacy.

Does point-of-care CYP2C19 genotyping identify loss-of-function variants and alter management in patients with IS/TIA or requiring angioplasty?

Population

77 patients with IS/TIA on clopidogrel or requiring angioplasty at a tertiary stroke centre

Comparison

Point-of-care CYP2C19 genotyping using Genomadix Cube

Design

Pilot observational study

Follow-up

Three months

Key result

Point-of-care CYP2C19 genotyping identified loss-of-function variants in 26% of stroke or TIA patients, but demonstrated a 20% test failure rate that may reduce cost efficiency.

Authors

NWNicholas Wetherall

Discussion

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Overview

Caution with empiric clopidogrel in ischaemic stroke; extends pharmacogenetic evidence into Level 1 European guidelines.

Key Points

  • To evaluate the clinical utility of point-of-care CYP2C19 genotyping in stroke prevention.
  • Conducted point-of-care CYP2C19 genotyping in 77 participants at a tertiary stroke center.
  • Included individuals with ischaemic stroke or transient ischaemic attack, and those requiring angioplasty.
  • Used Genomadix Cube for genotyping and assessed clinical outcomes at three-month follow-up.
  • 26% (n=19) of participants carried CYP2C19 LOF variants, showing clopidogrel resistance.
  • No recurrent ischaemic strokes or TIAs were observed in clopidogrel-resistant participants by three-month follow-up.
  • Observed a 20% failure rate in point-of-care testing, impacting cost efficiency.

Study Design

Type

Observational (n=77)

Multicenter

No

Structured PICO

Does point-of-care CYP2C19 genotyping identify loss-of-function variants and alter management in patients with IS/TIA or requiring angioplasty?

P
Population
77 participants at a UK tertiary stroke centre presenting with ischaemic stroke (IS) or transient ischaemic attack (TIA) despite clopidogrel secondary prevention, or requiring angioplasty.
I
Intervention
Point-of-care (POC) CYP2C19 genotyping using Genomadix Cube
O
Outcome
Identification of CYP2C19 loss-of-function variants and test failure rate

Point-of-care CYP2C19 genotyping in stroke patients successfully identifies clopidogrel resistance to guide alternative therapy, though high test failure rates present an implementation challenge.

Limitations

  • Relatively high POC test failure rate, which may reduce overall cost efficiency
  • Relatively high POC test failure rate which may reduce overall cost efficiency

Cite This Study

Nicholas Wetherall (2026) conducted an observational in Ischaemic stroke or transient ischaemic attack (n=77). Point-of-care CYP2C19 genotyping was evaluated on CYP2C19 loss-of-function (LOF) variants (*2 or *3) prevalence. Point-of-care CYP2C19 genotyping identified loss-of-function variants in 26% of stroke or TIA patients, but demonstrated a 20% test failure rate that may reduce cost efficiency.

synapsesocial.com/papers/69fd7eb0bfa21ec5bbf06f76https://doi.org/10.1093/esj/aakag023.1196
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