Abstract Background: A diverse set of clonal hematopoietic illnesses known as myelodysplastic syndrome (MDS) have decreased survival rates, risk factors for leukemia transformation, and a variety of clinical manifestations. This makes the clinical relevance of an accurate diagnosis and risk classification of MDS patients. Objectives: Evaluation of serum levels of reactive oxygen species (ROS) and some antioxidants in patients with MDS. Materials and Methods: A total of 20 healthy individuals as the control group (12 men and 8 women), and 45 MDS patients (25 males and 20 females). MDS samples were collected from several Iraqi locations. The serum oxidative stress level was assessed by measuring ROS using an automated colorimetric method. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) in serum were determined using an enzyme-linked immunosorbent assay approach. Moreover, glutathione reductase (GR) was measured by using Ellmam’s reagents methods. Results: The ages of the participants ranged from 20 to 65 years, with mean ages of 38 ± 4.43 and 40 ± 5.93 years for patients and healthy people, respectively. The prevalence was higher in males than females among patients (62.5% and 37.5%, respectively). ROS levels were found to be significantly higher in the patient group (33.64 µmol/L) than that of control (16.31 µmol/L) ( P = 0.041). Antioxidants, such as SOD and GPx, levels were significantly higher in MDS patients (12.9 and 78.65 U/L, respectively) compared with the control group (2.04 and 10.09 U/L, respectively; P < 0.05). Serum level of GR deceased in MDS patients compared with control groups (43.26 and 71.46 pg/mL, respectively; P = 0.014). Conclusion: ROS, GPx, and SOD increased in patients compared with control in contrast to GR that decreased in patients so all these indicators were significantly associated with MDS.
Zaid et al. (Thu,) studied this question.