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May 9, 2026Oikos0 citationsOpen Access

Calibrating p‐values in ecology: a practical framework for integrating prior plausibility into statistical inference

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RGRafael Dettogni GuarientoUniversidade Federal de Mato Grosso do SulAGAnderson da Rocha GrippUniversidade Federal do Rio de JaneiroACAdriano CalimanUniversidade Federal do Rio Grande do Norte

Key Points

  • The research aims to improve the interpretation of p-values by integrating prior plausibility into statistical inference in ecology.
  • Developed a framework for p-value calibration using Bayes factors to assess evidential strength.
  • Applied the framework to trophic cascade experiments as a practical example.
  • Outlined a structured approach for assigning prior probabilities based on existing theory and data.
  • Demonstrated that identical p-values can indicate different posterior probabilities based on prior plausibility.
  • Provided tools for incorporating prior evidence in p-value analysis effectively.
  • Showed potential for reduced false-positive claims and a stronger alignment of statistical output with ecological theory.

Abstract

Misinterpretation of p‐values, coupled with insufficient consideration of the prior plausibility of ecological hypotheses, leads to overconfident and often unreliable inference in ecological research. To address this issue, we present a methodological framework for p‐value calibration that reinterprets conventional p‐values through minimum Bayes factors to quantify evidential strength given the prior probability of a hypothesis. Using trophic cascade experiments as a case study, we demonstrate how identical p‐values can correspond to different posterior probabilities depending on prior plausibility, and we provide practical tools for applying calibration in ecological analyses. This approach requires only standard p‐values and a qualitative or quantitative assessment of prior evidence, making it readily applicable to both existing and new studies. We also outline a structured framework for assigning prior probabilities based on theoretical and meta‐analytic information, and show how calibration can help reduce false‐positive claims, promote replication, and better align statistical inference with ecological theory. This work promotes a pragmatic, easily implemented method that bridges current frequentist practice and Bayesian reasoning, with the goal of strengthening the reliability and interpretation of ecological research.

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Cite This Study

Guariento et al. (2026) studied this question.

synapsesocial.com/papers/69fecfafb9154b0b828769f0https://doi.org/10.1002/oik.12167
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