432 Early attachment calibrates stress biology signatures in salivary DNA methylation aging

Objectives/Goals: Test whether early childhood attachment security relates to epigenetic aging during adolescence, evaluate consistency across clocks, and examine heterogeneity for targeting. Methods/Study Population: Adolescence is a high-stakes period for stress biology. DNA methylation clocks condense CpG information into indices of cellular and system-level aging that relate to stress load and risk profiles. This study examines a population-based cohort with saliva DNA methylation at ages 9 and 15 and 5 clocks. Prespecified longitudinal regression and ANCOVA assess associations and incremental value beyond age 9 clocks. Models adjust for prespecified social and technical covariates; covariate missingness is handled with multiple imputation. False discovery rate control is applied within each wave. Sensitivity checks vary covariates and scaling. Results/Anticipated Results: Findings show directionally consistent protective signals linking early attachment security to youth DNA-based aging. The pattern concentrates by age 9, guiding precision child health efforts toward early relational supports and low-burden, saliva-based screening that is feasible in diverse community and clinical settings. Discussion/Significance of Impact: Results support a pathway to intervention. Attachment screening paired with saliva methylation clocks can identify who benefits from relational supports, when to intervene, and how to set decision thresholds. The approach fits pediatric workflows, guides stratification in attachment-focused trials, and is scalable to external validation.