Label-Free Fluorometric Split Aptasensor for the Specific Detection of Uric Acid from Xanthine and Hypoxanthine

values of these split aptamers, as measured by thioflavin T (ThT) fluorescence, were 48.78 μM and 38.72 μM, respectively. To facilitate sensor development, the kinetic and thermodynamic characteristics of these aptamers were evaluated. The results indicated that Apt2-T4-T3/Apt2-T5 binds rapidly to UA, and the melting temperature (Tm) of Apt2-T4-T3/Apt2-T5 was determined to be 40.48 ± 0.18 °C. Finally, we developed a label-free fluorometric split aptasensor based on Apt2-T4-T3/Apt2-T5, which achieved a detection limit of 0.60 μM and exhibited a strong linear response within the UA concentration range of 3.15-200 μM. Subsequently, the aptasensor was applied to detect UA in real urine samples.