May 9, 2026Journal of Agricultural and Food Chemistry0 citations
Structure-Guided Engineering of UGT94B1 M0 Enhances Thermostability and Enables Efficient Rebaudioside D Biosynthesis via Coupled UDP-Glucose Regeneration
This research aims to improve the thermostability of UGT94B1 for better glycoside biosynthesis.
Engineered UGT94B1 M0 for enhanced thermostability via structural guidelines.
Focused on the regeneration of UDP-glucose to boost glycosylation efficiency.
Demonstrated significant improvement in thermostability of UGT94B1 M0.
Achieved effective biosynthesis of rebaudioside D using engineered enzyme.
Abstract
SuSy. This work establishes a generalizable strategy for thermostability engineering of UDP-glycosyltransferases toward efficient and sustainable glycoside biosynthesis.