A Statistical Study on the Impact of Crab Shell Powder Alcoholic Extract on the Growth and Proliferation of Human Cancer Cells

A laboratory experiment was conducted at the Iraqi Center for Cancer and Medical Genetics Research at Mustansiriya University in Baghdad in early 2005 to investigate the effects of an alcoholic extract derived from crab shell powder on the proliferation and growth of human cancer cells in vitro. The study followed a concentration- and time-dependent format, incorporating two main factors: time at three levels (24, 48, and 72 hours) and extract concentration at three levels (100 mg, 2600 mg, and 5100 mg). The primary variable, cancer cell density (count), was measured using a Microplate Reader at a wavelength of 492 nanometers. The data were statistically analyzed as a two-factor factorial experiment according to a Completely Randomized Design (CRD). The findings revealed highly significant effects of both time and extract concentration, as well as their interaction, on the studied trait. Furthermore, response level analysis indicated a significant linear effect and a highly significant quadratic effect for the time factor, while the concentration factor showed a highly significant linear effect and a significant quadratic effect. Additionally, the analysis identified a significant interaction between the linear components of time and concentration, alongside a highly significant interaction between the quadratic component of time and the linear component of concentration.